Optimization of Extraction and Alcohol Precipitation process for compound recipe HuZhang Granule

Objective To optimize water extraction and alcohol precipitation process for compound recipe Hu-zhang granule.Methods The amount of water extracts and polydatin were used as comprehensive evaluation indexes,orthogonal test was used to optimize extraction technology with the amount of water,decocting times and decocting time as factors.Single-factor test was used to optimize alcohol precipitation process with the concentration of alcohol precipitation and relative density of extract were chosen as factors.Results Optimum water extraction process was as follows: decocted 3 times with 12,10,10 times the amount water,0.5 h each time; optimum alcohol precipitation process was: concentrated extraction liquid to its relative density 1.13( 70℃),added alcohol until the concentration of ethanol at 80%,stilled 24 h.Conclusion These optimized of water extraction and alcohol precipitation process could ensure effective ingredient saved to the maximum and achieve good impurity effect.The experiment result can provide basic and instruction data for the ascertainment of water extraction technology of polydatin.