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2007•Journal of Yangzhou UniversityRequires access

Development and characterization of monoclonal antibody specific to nucler protein of avian influenza virus

Yuelong Liu

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Abstract

Five monoclonal antibodies(Mabs) to nucler protein of avian influenza virus (AIV) were developed by fusion between SP2/0 cells and the spleen cells from BALB/c mice immunized with H9 subtype AIV.Specificity of these Mabs were identified by immunofluorescent assay(IFA) and enzyme link immunosorb assay (ELISA).These five Mabs could react with all viruses of AIV-H9 strains in tests,but didn't react with Newcastle disease virus,REV and infectious bursa disease virus.Only a 60 ku protein band of AIV-H9 was recongnized by the Mabs in Western blotting.This suggested that the five Mabs,which were named as AIV-NP-2C3,AIV-NP-6A5,AIV-NP-3H9,AIV-NP-7B4 and AIV-NP-2H4 respectively,were specific against the nucler protein of avian influenza viruses.All avian influenza viruses could be dectected with these Mabs in IFA and ELISA.These results indicate that these Mabs will be potentially useful as immunodiagnostic reagents in future.

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What this paper is about

Five monoclonal antibodies(Mabs) to nucler protein of avian influenza virus (AIV) were developed by fusion between SP2/0 cells and the spleen cells from BALB/c mice immunized with H9 subtype AIV.Specificity of these Mabs were identified by immunofluorescent assay(IFA) and enzyme link immunosorb assay (ELISA).These five Mabs could react with all viruses of AIV-H9 strains in tests,but didn't react with Newcastle disease virus,REV and infectious bursa disease virus.Only a 60 ku protein band of AIV-H9 was recongnized by the Mabs in Western blotting.This suggested that the five Mabs,which were named as AIV-NP-2C3,AIV-NP-6A5,AIV-NP-3H9,AIV-NP-7B4 and AIV-NP-2H4 respectively,were specific against the nucler protein of avian influenza viruses.All avian influenza viruses could be dectected with these Mabs in IFA and ELISA.These results indicate that these Mabs will be potentially useful as immunodiagnostic reagents in future.

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Available abstract

Five monoclonal antibodies(Mabs) to nucler protein of avian influenza virus (AIV) were developed by fusion between SP2/0 cells and the spleen cells from BALB/c mice immunized with H9 subtype AIV.Specificity of these Mabs were identified by immunofluorescent assay(IFA) and enzyme link immunosorb assay (ELISA).These five Mabs could react with all viruses of AIV-H9 strains in tests,but didn't react with Newcastle disease virus,REV and infectious bursa disease virus.Only a 60 ku protein band of AIV-H9 was recongnized by the Mabs in Western blotting.This suggested that the five Mabs,which were named as AIV-NP-2C3,AIV-NP-6A5,AIV-NP-3H9,AIV-NP-7B4 and AIV-NP-2H4 respectively,were specific against the nucler protein of avian influenza viruses.All avian influenza viruses could be dectected with these Mabs in IFA and ELISA.These results indicate that these Mabs will be potentially useful as immunodiagnostic reagents in future.

Key concepts: Virology, Monoclonal antibody, Avian influenza virus, Biology, Influenza A virus subtype H5N1, Virus, Newcastle disease, Spleen

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