Extraction and Bioassay of the Toxin Produced by Cercospora fuligenae
Fumei Wang
Abstract
Fumei Wang
Abstract
In this article three methods of the extraction of toxin from Cercospora fuligenae were experimented and the results showed activeted carbon,organic solvent and grade-precipitating with ammonium sulfate can be extracted and grade-precipitating with ammonium was the best effective and convenient method.Bioassay results indicated that the toxin could cause wilt of seedlings and leave of tomato.The results of dialyzation and heating stability showed that the toxin was a kind of big molecule compound.Meanwile the results indicated the toxin was powerful to resist high temperature.
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In this article three methods of the extraction of toxin from Cercospora fuligenae were experimented and the results showed activeted carbon,organic solvent and grade-precipitating with ammonium sulfate can be extracted and grade-precipitating with ammonium was the best effective and convenient method.Bioassay results indicated that the toxin could cause wilt of seedlings and leave of tomato.The results of dialyzation and heating stability showed that the toxin was a kind of big molecule compound.Meanwile the results indicated the toxin was powerful to resist high temperature.
Key concepts: Bioassay, Toxin, Extraction (chemistry), Ammonium sulfate, Solvent, Ammonium, Chemistry, Solvent extraction