Cloning,bioinformatics analysis and expression analysis of GpAPX during dehydration and rehydration in Grimmia pilifera

In this study,a full-length cDNA sequence of a ascorbate peroxidase(APX)gene named GpAPX was cloned from Grimmia pilifera(GenBank accession No.GU989311)using rapid amplification of cDNA ends(RACE).The full-length cDNA of GpAPX was 1 115 bp with a 771 bp open reading frame(ORF)which encoded 256 amino acids and containing a 116 bp 5′UTR and a 228 bp 3′UTR.Sequence analysis showed that GpAPX belonged to the peroxidase protein families.GpAPX protein sharing 70.2%-89.7% homology with APX proteins from other plants.QRT-PCR analysis suggested that the expression of GpAPX gene was induced in both dehydration and rehydration.The results demonstrated that the GpAPX protein may be involved in drought resisitance,and play an important role in Grimmia pilifera through responses to drought stress.