Optimization of the extraction conditions for volatile oil from Compound Yixin Granules andβ-cyclodextrin inclusion technology

Objective To optimize the extraction conditions andβ-cyclodextrin(β-CD)inclusion process for volatile oil from Compound Yixin Granules.Methods Volatile oil was extracted by steam distillation method.The extracting technology was optimized by using the orthogonal design,and the yield of volatile oil was used to evaluate the extraction process;theβ-CD inclusion complex of volatile oil was prepared by saturated aqueous solution method,and the orthogonal test was used to optimize the inclusion technology with ratio of volatile oil toβ-CD,inclusion time and temperature as factors,and inclusion yield and volatile oil inclusion rate as indexes.Results The optimized volatile oil extraction conditions were to add 10 folds water into the mixture of crude drugs,and to extract the herbal medicine for 5hwith previous soaking for 8hwith water.The optimized inclusion procedure was as follows:the proportion of volatile oil andβ-CD was 1∶10and stirring for 2hat 40℃.The inclusion complex of volatile oil-β-CD was stable to light,heat and humidity.Conclusion The optimized extraction and inclusion conditions were reasonable and feasible.