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2008•ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSISRequires access

Studies on Liquid-state Fermentation Culture Medium of Cellulase from Aspergillus niger No.5.1

Shang Xiao-xian

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Abstract

The production technology of cellulase by liquid deep fermentation was studied with Aspergillus niger No.5.1.Its optimal composition of culture medium for enzyme secretion by liquid-state fermentation was cellulose powder 4%,KNO3 3%,PEG 0.1%,and initial pH 6.5 when the cultivating condition was 25 mL medium in 250 mL flask,inoculating 2.5 mL of 1 day of seed liquid,incubation temperature 32 ℃,fermentation for 5 days.This paper provides some theoretical bases for the industrial production and application of cellulase.

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The production technology of cellulase by liquid deep fermentation was studied with Aspergillus niger No.5.1.Its optimal composition of culture medium for enzyme secretion by liquid-state fermentation was cellulose powder 4%,KNO3 3%,PEG 0.1%,and initial pH 6.5 when the cultivating condition was 25 mL medium in 250 mL flask,inoculating 2.5 mL of 1 day of seed liquid,incubation temperature 32 ℃,fermentation for 5 days.This paper provides some theoretical bases for the industrial production and application of cellulase.

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Available abstract

The production technology of cellulase by liquid deep fermentation was studied with Aspergillus niger No.5.1.Its optimal composition of culture medium for enzyme secretion by liquid-state fermentation was cellulose powder 4%,KNO3 3%,PEG 0.1%,and initial pH 6.5 when the cultivating condition was 25 mL medium in 250 mL flask,inoculating 2.5 mL of 1 day of seed liquid,incubation temperature 32 ℃,fermentation for 5 days.This paper provides some theoretical bases for the industrial production and application of cellulase.

Key concepts: Cellulase, Aspergillus niger, Fermentation, Liquid culture, Food science, Cellulose, Liquid medium, Solid-state fermentation

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