Ultrasound-Assited Extraction and Antioxidation of Elaeagnus Angustifolia Seed Oil

This paper was designed to systematically research antioxidant activity from Elaeagnus angustifolia seed oil,which was extracted from Elaeagnus angustifolia seed with ultrasonic wave assistance.On the basis of single factor experiment,the process conditions of the ultrasonic-assisted extraction were optimized by using orthogonal experiment design.Results:The effects of affecting factors rank as extraction temperature extraction time ultrasonic power solvent/material ratio,and the optimum conditions of the extraction were extraction time 30 min,extraction temperature 30 ℃,ultrasonic frequency 120 W and solvent/material ratio 1 ∶ 6(g/mL).The extraction rate was 26.07 % with these conditions.Elaeagnus angustifolia seed oil which extracted with ultrasonic wave assistance had strong antioxidant activitie.The scavenging rate against hydroxyl radicals(.OH) of 10 mg/mL Elaeagnus angustifolia seed oil was 87.05 %;The scavenging rate against DPPH of 8 mg/mL Elaeagnus angustifolia seed oil was 52.36 %;The scavenging rate against superoxide anion(O2-.)of 8 mg/mL Elaeagnus angustifolia seed oil was 59.55 %.