Effects of Saikosaponin D on Nitric Oxide and JNK Phosphorylation of SH-SY5Y Cells

Objective To investigate the effects of Saikosaponin d(SSd)on nitric oxide(NO)and c-Jun N-terminal kinase1/2(JNK1/2) expressions in SH-SY5Y cells(SH-SY5Y). Methods SH-SY5Y cells were cultured in vitro firstly,then SSd was dissolved in DMSO,and the cultures were pretreated individually with 0 to 10 μmol/L SSd for 48 h,then the effect of different concentrations of SSd on the concentration of NO in the cells′ culture was detected by NO Test Kit. At the same time,changes of expression level of t-JNK and p-JNK proteins were analyzed by Western blot. Results Compared with the control group,a significant inhibition of NO was observed in SH-SY5Y cells after treatment with SSd from 4 to 10 μmol/L groups for 48 h,and the content of NO was minimum in 4 μmol/L SSd groups(P0.05). It also showed that SSd from 4 to 10 μmol/L groups increased the expression levels of p-JNK in SH-SY5Y cells,and the expression level of pJNK was the highest in 10 μmol/L SSd group(P0.01). Conclusion These results demonstrate that a certain concentration of SSd can reduce the NO expression and significantly increase the JNK protein phosphorylation levels in SH-SY5Y cells.