Protective Effect of Saikosaponin B2 on Damage of Cultured SH-SY5Y Cells in Vitro Introduced by Hydrogen Peroxide

Objective To investigate the effect of saikosaponin B2 on the damage of cultured SH-SY5Y cells. Methods 10% calf serum including volume fraction 0.05, 0.10, 0.20 saikosaponin B2 (10−4mol·L−1) were added respectively into the SH-SY5Y cells, which were then treated with 140 μmol· L−1 hydrogen peroxide(H2O2). 10% calf serum group and blank serum without H2O2-treated group were as the model group and the control group. The effect of saikosaponin B2 was observed by morphological identification, colorimetric MTT assay. Results Both saikosaponin B2 of 10−6mol·L−1 and 2 × 10−6mol·L−1 can relieve the damage of SH-SY5Y cells and increase the survival of the cells. Conclusion saikosaponin B2 can protect the cultured SH-SY5Y cells from damage induced by H2O2. Disclosure of interest The authors have not supplied their declaration of competing interest.