Improvement on quality standard for Yinhuang Injection

Objective: To improve the quality standard of Yinhuang Injection. Methods: HPLC was adopted for the determination of the chlorogenic acid and baicalin at the same time. Agilent-C18 (150 mm×4.6 mm,5 μm) was used,the mobile phase was composed of methanol-0.4% phosphoric acid(30∶60)with the flow rate of 1.0 ml/min,the detective wavelength was set at 327 nm for chlorogenic acid and 276 nm for baicalin,the column temperature was maintained at 30℃. Results: The linear range of chlorogenic acid was 0.010 83-0.108 30 mg/ml (r=0.999 44),the average recovery was 100.24% with RSD of 1.54%. The linear range of baicalin was 0.015 15-0.151 50 mg/ml (r=0.999 99),the average recovery was 100.48% with RSD of 0.97%. Conclusion: The method is simple,accurate,and can be used for the quality control of Yin-huang Injection.