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2008•Journal of Pathogen BiologyRequires access

Cell apoptosis of U937 induced by Pseudomonas aeruginosa infection

Wenshu Chai

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Abstract

Objective To investigate the relationship between Pseudomonas aeruginosa(PA) and the apoptosis of U937 cell. Methods U937 cell apoptosis was induced by PA in groups of U937: PA= 1∶10,1∶20,1∶50 and 1∶100,respectively,and detected with Hochest 33258 fluorochrome staining and Annexin V/PI assay. Results When the ratio between cell and bacterium was 1∶10,the apoptotic cells were found in all the groups.Hoechst 33258 fluorochrome staining and Annexin V/ PI assay showed that the percentage of apoptotic U937 for(11.67±1.75)% and(11.68±2.58)%,and the percentage of apoptotic cells was raised with the increase of the concentration of PA.Conclusion PA can induce the U937 cells apoplosis in a dose-dependent manner.

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Objective To investigate the relationship between Pseudomonas aeruginosa(PA) and the apoptosis of U937 cell. Methods U937 cell apoptosis was induced by PA in groups of U937: PA= 1∶10,1∶20,1∶50 and 1∶100,respectively,and detected with Hochest 33258 fluorochrome staining and Annexin V/PI assay. Results When the ratio between cell and bacterium was 1∶10,the apoptotic cells were found in all the groups.Hoechst 33258 fluorochrome staining and Annexin V/ PI assay showed that the percentage of apoptotic U937 for(11.67±1.75)% and(11.68±2.58)%,and the percentage of apoptotic cells was raised with the increase of the concentration of PA.Conclusion PA can induce the U937 cells apoplosis in a dose-dependent manner.

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Available abstract

Objective To investigate the relationship between Pseudomonas aeruginosa(PA) and the apoptosis of U937 cell. Methods U937 cell apoptosis was induced by PA in groups of U937: PA= 1∶10,1∶20,1∶50 and 1∶100,respectively,and detected with Hochest 33258 fluorochrome staining and Annexin V/PI assay. Results When the ratio between cell and bacterium was 1∶10,the apoptotic cells were found in all the groups.Hoechst 33258 fluorochrome staining and Annexin V/ PI assay showed that the percentage of apoptotic U937 for(11.67±1.75)% and(11.68±2.58)%,and the percentage of apoptotic cells was raised with the increase of the concentration of PA.Conclusion PA can induce the U937 cells apoplosis in a dose-dependent manner.

Key concepts: U937 cell, Annexin, Apoptosis, Pseudomonas aeruginosa, Pi, Staining, Molecular biology, Cell

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