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2010•CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION)Requires access

Determination of Microcystins in Aquatic Environment with High Performance Liquid Chromatography

Liang Li

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Abstract

The determination method for microcystins (MCs) in aquatic environment with high-performance liquid chromatography was improved. The results showed that good effects on the rinse of impurity was obtained by using carbon tetrachloride-10% ethanol-10% acetone as the eluate, and all the MCs in XAD-2 resin column could be eluted by 90% methanol-water solution. In the experiment, MC-RR and MC-LR could be totally separated at isocratic elution with mobile phase V(0.1% trifluoroacetic acid)∶V(methanol)=42∶58 and the recoveries both reached 90%. The detection limits for both of the MCs were 0.1 μg/L after the water samples were condensed 6000 times. The detection method is simple, sensitive and fast.

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What this paper is about

The determination method for microcystins (MCs) in aquatic environment with high-performance liquid chromatography was improved. The results showed that good effects on the rinse of impurity was obtained by using carbon tetrachloride-10% ethanol-10% acetone as the eluate, and all the MCs in XAD-2 resin column could be eluted by 90% methanol-water solution. In the experiment, MC-RR and MC-LR could be totally separated at isocratic elution with mobile phase V(0.1% trifluoroacetic acid)∶V(methanol)=42∶58 and the recoveries both reached 90%. The detection limits for both of the MCs were 0.1 μg/L after the water samples were condensed 6000 times. The detection method is simple, sensitive and fast.

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Available abstract

The determination method for microcystins (MCs) in aquatic environment with high-performance liquid chromatography was improved. The results showed that good effects on the rinse of impurity was obtained by using carbon tetrachloride-10% ethanol-10% acetone as the eluate, and all the MCs in XAD-2 resin column could be eluted by 90% methanol-water solution. In the experiment, MC-RR and MC-LR could be totally separated at isocratic elution with mobile phase V(0.1% trifluoroacetic acid)∶V(methanol)=42∶58 and the recoveries both reached 90%. The detection limits for both of the MCs were 0.1 μg/L after the water samples were condensed 6000 times. The detection method is simple, sensitive and fast.

Key concepts: Chemistry, Chromatography, Elution, Trifluoroacetic acid, Methanol, Acetone, High-performance liquid chromatography, Detection limit

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