Effect of Shenmai Injection on Chondrocyte Proliferation Induced by Interleukin-1 Beta in Rabbits

Objective:To observe the effect of Shenmai Injection on chondrocyte proliferation induced by interleukin-1 beta in rabbits.Methods:The degenerated chondrocyte model was induced established by IL-1 beta inducing rabbit chondrocyte in vitro.The model cells were divided into the blank group,the model group,1% Shenmai group,5% Shenmai group and 10% of Shenmai group.The blank group was cultured by DMEM with 10% fetal bovine serum,and the model group was cultured by DMEM with 10ng/mL IL-1 beta.Shenmai groups of 1%,5% and 10% were cultured by DMEM with 10ng/mL IL-1 beta for 24h firstly,and then was culture DMEM with Shenmai injection of 1%,5%,10% respectively in culture medium.Chondrocyte proliferation was detected by MTT assay.Results:Absorbance(OD) value in the model group was decreased(P 0.01compared with that in the blank group).It refered that IL-1β can inhibits chondrocyte proliferation to establish the degenerated chondrocyte model.The differences of absorbance(OD) value among all Shenmai groups and the model group were significant differences(P 0.01).It refered that Shenmai injections of different concentration can obviously promote the degenerated chondrocyte proliferation,the proliferation effect being positively correlated with the concentration.Conclusion:Shenmai injection can obviously promote the chondrocyte proliferation induced by IL-1β in rabbits,which might be the mechanism of Shenmai injection to prevent and treat joint degeneration.