The Effects of Jinbianqufengyin on Cytokine of Rats with Collagen-induced Arthritis

Objective To investigate the therapeutic effect of JBY on a rat model of Collagen-induced Arthritis(CIA).Methods 70 SD rats were randomly divided into experimental group,normal control group,and MTX group.Arthritis in rats was induced by injection with mixture of II collagen,incomplete Freund′s adjuvant and Acetic acid in left feet.Pathological changes and swelling scores were observed.TNF-α,IL-1β,and MMP-3 in plasma were also detected to evaluate the effect of JBY.Results 1.Polyarthritis occurred in 98 percent of rats 14 days after injecting type II collagen with Eph-type incomplete adjuvant into left feet,companied with inflammatory nodules and other systemic signs.2.In the groups of JBY-treated CIA rats,the symptoms and signs of arthritis were relieved,with decreased arthritis scores and shortened course of arthritis(P0.01).3.The soft tissue around joints of CIA rats showed apparent swelling.And the inflammatory responses with a lot of lymphocytes infiltration and lining synoviocytes proliferation in the synovial tissue of joint in CIA rats were observed under microscopic examination.However,the pathological changes in JBY treated CIA rat were fewer than those in non-JBY-treated ones.4.The levels of TNF-α,IL-1β,MMP-3 in JBY-treated CIA rat sera of cultured JBY-treated CIA rat synoviocytes were much lower than those in controls(P0.05 and 0.01 respectively).JBY can significantly improve the conditions of pathological changes and joint swelling.It can also decrease the levels of TNF-α,IL-1β,and MMP-3 in plasma.Conclusion The effective regulation of cytokines IL-1β,TNF-α,MMP-3 is perhaps one of the mechanisms of JBY in treating rheumatoid arthritis.