Effects of Ligustrazine and IFN-γ on the levels of GATA-3,IL-4 and IL-5 in asthmatic rats

Objective To explore the effects of ligustrazine and IFN-γ on the levels of GATA-3,IL-4 and IL-5 in asthmatic rats.Methods Fifty SD rats were randomly divided into experimental groups(10 in each):normal group,control group,ligustrazine treatment group,IFN-γ treatment group and ligustrazine+IFN-γ treatment group.The asthmatic rat model was established by inhalation of ovalbulium repeatedly.The levels of IL-4 and IL-5 in BALF and the expression of GATA-3 in the lung were measured.Results(1) The number of total cells and EOS in the BALF were high in the control group,decreased in the treatment groups with significant difference among the ligustrazine+IFN-γ treatment group,the ligustrazine treatment group and the IFN-γ treatment group(P0.05),respectively.(2) The levels of IL-4 and IL-5 in the BALF were high in the control group,decreased in the normal group and the treatment groups with significant difference among the ligustrazine+IFN-γ treatment group,the ligustrazine treatment group and the IFN-γ treatment group(P0.05),respectively.(3) The thicknesses of WA/Pi and AS/Pi were large in the control group,decreased in the normal group and the treatment groups of the ligustrazine+IFN-γ treatment group,the ligustrazine treatment group and the IFN-γ treatment group(P 0.05),respectively.(4) The expression of GATA-3 was strong in airway and lung in the control group,weak in the ligustrazine treatment group and in the IFN-γ treatment group,and none in the ligustrazine+IFN-γ treatment group.Conclusion The ligustrazine+IFN-γ can decreased the expression of GATA-3 and the levels of IL-4 and IL-5,and inhibit inflammation in the airway and lung in asthmatic rats.