Screening of Endophytic Fungi from Mint and Its Preliminary Study

[Objective] The aim was to screen the endophytic fungi that could generate the active material.[Method] With tissue isolation,3 different endophytic fungi of P-1,P-2 and P-3 were isolated from mint,which were cultured in different pH gradient,and their antibacterial activities were studied by using the methods of filter paper and confront culture.[Result] 3 strains of endophytic fungi had growth peak when the pH value was 6.5-7.5,and could grow within pH of 4.0-8.5.Their acid-alkali adaptability was strong,in which the P-3 adaptability was the strongest.P-1and P-2 had the stronger inhibition to the Bacillus subtilis,Escherichia coli and Staphylococcus aureus,and P-3 only had the little inhibition to Escherichia coli.The 3 endophytic fungi all had inhibitory effect on Podosphaera leucotrlcha,Alternaria mali,and Gymnosporangium yamadae Miyabe,in which,P-1 had the better inhibitory ability.[Conclusion] The endophytic fungi with antibacterial activity was obtained in this study.