Antimicrobial Activity of Endophytic Fungi Isolated from hysalis angulata L. Plant

Some endophytic fungi live in plant tissue and does not caused any damage on their host plant. The endophytic fungi could produce antimicrobial secondary metabolites. Some endophytic fungi have been isolated from a medicinal plant Physalis angulata, i.e: Penicillium verrucosum, Colletotrichum alienum, Fusarium subglutinans, Aspergillus nidulans, Mycelia sterilia 1, Mycelia sterilia 2, and Rhizoctonia sp. The purpose of this research are: 1) to examine the antimicrobial activity of each endophytic fungi species toward Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and, 2) to determine the endophytic fungi species that have highest antimicrobial activity. Each endophytic fungi isolates were inoculated in Potato Dextrose Broth (PDB) medium and shaked in 120 rpm, during 7 x 24 hours, then the broth culture centrifugated in the rate of 3000 rpm for 10 minutes. The supernatants from each endophytic fungi were treat to the bacteria to know the antimicrobial activity by agar diffusion method. Afterwards the antibaterial effect of each endophytic fungi species were measured. Then the highest antimicrobial effect were determined. The research results shows that: 1) each endophytic fungi species have antimicrobial activity towards B. subtilis, E. coli, S. aureus, and; 2) Penicillium verucossum secondary metabolites have the highest antimicrobial activity.