Oxidative stress and inflammation resulting in metabolic syndrome in rats dunng chronic intermittent hypoxia

ObjectiveTo study the mechanism of oxidative stress and inflammation resulting in metabolic syndrome in rats during chronic intermittent hypoxia.MethodsTo establish a chronic intermittent hypoxia(CIH)animal model inrats, in order to mimic the intermittent hypoxia of obstructive sleep apnea-hypopnea syndrome(OSAHS)in humans. 50 healthy male SD rats were randomly assigned to two groups: the control group(10) and the CIH group(40). The rats in CIH group were placed in all animal chamber subjected to chronic intermittent hypoxia(nadir ambient oxygen concentration 6%~7%, maximum ambient oxygen concentration 20%~21%, Continuing respectively to5~7 second) for 8 hours per day(from 9AM to 5PM). The rats in control group were placed in the same animal chamber with out chronic intermittent hypoxia. The experiment lasted for 12 weeks.After the experiment, we measured the level of MDA,SOD and hs-CRP in plasma by ELISA.And measure various laboratory indices of metabolic syndrome such as blood sugar levels、triglyceride(TG)、total cholesterol(TC)、blood pressure.ResultsAlong with the time prolonged intermittent hypoxia, the rising risk factors of MS, and the level of of supemxide dismutase(SOD), malondia Jdehyde(MDA) and the hs-CRP, there were significant differences between the two groups.ConclusionOxidative stress and inflammation may be important in patients of OSAHS resulting in metabolic syndrome.