Protective effects of ischemic preconditioning on reperfusion injury in isolated rat heart

Objective To study the protective effect of ischemic preconditioning on myocardial contractile function in isolated ischemia/reperfusion rat. Method The model of Langendorff perfused isolated rat heart was used to study the effects of ischemic preconditioning on LVEDP, DP, +dp/dt, -dp/dt, CF, protein contents, activities of LDH and SOD, concentration of MDA and ATP level pre- and post-ischemia/reperfusion. Result Compared with the ischemia/reperfusion group, ischemic preconditioning enhanced the recovery of cardiac hemodynamics. During ischemia period, ischemic preconditioning significantly delayed the onset time of ischemic contraction. And it promoted the recovery of contractile function, ATP level and SOD activity, and alleviated leakage of intracellular lactee dehydrogenase and MDA product in myocardium during reperfusion period. Conclusion The ischemic preconditioning has protective effects on ischemia and reperfusion injury, and may play and important role in future clinic practice.