Application of the Multiplex PCR Detecting PCV2,PPV,PRV,PRRSV and CSFV

The established multiplex PCR of PCV2-PPV-PRV-PRRSV-CSFV was used to detect thirty-two clinical cases.These cases were collected from different areas of Shandong Province for the PCR detection.The results showed that the infection rate of PCV2,PPV,PRV,PRRSV and CSFV was 25.00%,0%,34.38%,71.88% and 3.13%,respectively.Twenty-five positive cases were detected and positive rate was 78.13%.The infection ratio of PRV-PRRSV double pathogens was 31.25% and the infection ratio of PCV2-PRV-PRRSV three pathogens was 25.00%.The single PCR was used as a control,and the result showed that the multiplex PCR accorded with the single PCR about 100%.It indicated the multiplex PCR had higher sensitivity.The multiplex PCR can be used as a rapid etiology diagnostic method to detect clinical diseases of porcine circovirus type 2 infection,porcine parvovirus disease,swine pseudorabies,porcine reproductive and respiratory syndrome and classical swine fever.