The Response of Rat's Hepatic Fibrosis to TGF-β Signalling

Basing on the model of rats hepatic fibrosis induced by carbon tetrachloride(CCl4)to investigate the effects of hepatic fibrosis on TGF-β signaling Methods:throughout the study male Wistar rats were used.Animals were randomly divided into two groups including a healthy control group(n=6),a model group(n=14).The model groups were injected intraperitoneally(ip) with 50% CCl4(2 mL/kg/2day) for six weeks,to induce rat liver fibrosis.the results were evaluated by detections of the mRNA level of TGF-β,Collagen type,Collagen type III,Fibronection,PAI-1 and TGF-β by semi-quantitative RT-PCR,the level of Glutamate-pyruvate transaminase(ALT)and glutamic-oxalo acetic transaminase(AST)detected by spectrophotometer following the protocol of the Kit.Results:compared with model group,in rats that model,the mRNA level of TGF-β,Fibronection,PAI-1,TGFβ,Smad7 and Collagen type 1,Collagen type 3 in liver tissues were significantly increased(P0.01),ALT and AST too.(P0.01).Conclusion:there is directed relationship between TGF-β signaling and rats hepatic.At the same time,the expression lever of TGF-β was increased in hepatic fibrosis.