Determination of Total Polyphenols in Potato Using Folin-Ciocalteus Method

An improved UV spectrophotometry method was developed for analysis of total polyphenols in potato. Folin-Ciocalteus method for total polyphenols determination in potato was studied. After a series of optimization experiments, the best determination procedure of Folin-Ciocalteus method was put forward, that is, 5.0 mL water, 1.0 mL coloration reagent, and 3.0 mL 7.5% sodium carbonate solution were added orderly into 1.0 mL extraction of sample or standard gallic acid, then blended, and measured at 765 nm after coloration for 2 h. Under this procedure, relative standard deviation of the method was 7.7%, and average recovery rate was 100%-108%.This method is applicable to conventional laboratory determination of total polyphenols in potato.