RETROVIRAL TRANSFER OF IL-2 GENE INTO HUMAN LEUKEMIA CELL LINE K562

Objective:To transduce pGEN/IL 2 into K562 cells and investigate it's expression.Methods:Packaging cell PA317 were transfected with pGEN/IL 2 by liposome mediated gene transfer method.K562 cells were coincubated with PA317/IL 2 cells.Integration of IL 2 in the genome,transcription of its mRNA and expression of its protein in the transfected K562 cells were assayed by Southern blot,RT PCR and IL 2 dependent cell line CTLL.Results:IL 2 cDNA was integrated into K562 genome sucessfully.IL 2 mRNA was transcripted and IL 2 protein was expressed of 100u/10 6 cells per day by CTLL cell assay in the supernatant,K562 cells grew slower in vivo and in vitro.Conclusion:Lipofectin mediated bicistronic retrovirus gene transduction method is efficient.We Successfully transduced human IL 2 gene into packaging PA317 and human leukemia cell line K562 with this method and expressed high level of IL 2.