CONSTRUCTION OF HUMAN IL-16 EUKARYOTIC EXPRESSION VECTOR AND ITS FUNCTION OF SWITCHING Th2 TYPE TUMOR CELLS

Objective:To construct human IL 16 eukaryotic expression vector,and analyse its function of switching Th2 type tumor cells.Methods:The human IL 16 cDNA was amplified by RT PCR from peripheral blood mononuclear cells,and cloned into the PUC 18 T vector.Then the eukaryotic expression vector PcDNA3 IL 16 containing IL 16 cDNA was constructed and identified.The Karpas T lymphoma cells were transfected with PcDNA IL 16.Cytokines IFNγ,IL 2,IL 4,IL 6,IL 13,TNFα and IL 16 were detected by RT PCR from the positive cell clones.Results:The sequence of cloned human IL 16 cDNA completely matched with that of human IL 16 cDNA in the gene bank.RT PCR indicated the IL 16 mRNA was highly expressed in the Karpas cells after the recombinant vector PcDNA3 IL 16 was transfected into Karpas T lymphoma cells.The cells were switched from the expression of Th2 cytokines to Th0 type.The biological activity through MTT assay showed the proliferation of Karpas cells were sharply inhibited.Conclusion:The eukaryotic expression vector PcDNA3 IL 16 containing IL 16 cDNA was successfully constructed.The cytokine pattern of Karpas T lymphoma cells can be changed from Th2 type to Tho type by transfecting PcDNA IL 16.These results suggest that is a useful method to transfect IL 16 gene into tumor cells for tumor biotherapy.