Dynamic Changes of Cell Wall-Degrading Enzyme and Effect during Coniothyrium minitans Parasiting on Sclerotinia sclerotiorum

The paper studied the dynamic changes of chitinases and β-1,3-glucanases and the effects on the mycelium and hypha tissue of Sclerotinia sclerotiorum during the course of Coniothyrium minitans parasited on Sclerotinia sclerotiorum.The results showed that the activities of chitinases and β-1,3-glucanases were increased obviously in the initial stages of the parasition of Coniothyrium minitans on Sclerotinia sclerotiorum.And the two enzymes had the characteristic of induced enzymes.But the two enzymes activity could be tested when there was no Sclerotinia sclerotiorum could be parasited by Coniothyrium minitans.The above results showed that chitinases and β-1,3-glucanases produced by Coniothyrium minitans had some composed enzymes.The mycelium and hypha tissue were cleared up largely on the maximal activity of the two enzymes,which showed that the mycelium and tissue cleared up and rotted more easily under the synergism of the chitinases and β-1,3-glucanases.