On Factors Affecting Enzymatic Activity of Chitinase Produced by Mycoparasite Coniothyrium minitans

Essential characters of chitinase produced by Coniothyrium minitans in mycoparasitism on Sclerotinia sclerotiorum were studied.The results showed that the activity of the chitinase was opti- mized under 50℃and pH 8.0,stable in the pH value range from 5.0 to 9.0,improved by some metal cations,including Mg~(2+),Fe~(2+),Ca~(2+),Ba~(2+)and Mn~(2+),inhibited by Zn~(2+)and Cu~(2+),and increased by boric acid at 25～75 mmol/L and oxalic acid at 1～4 mmol/L.Importance of these factors affecting the chiti- nase activity and their potential applications in biological control were also discussed.