Effect of Huazheng Koufuye on expression of mutant p53 and plasma endothelin in cancerbearing mice

Objective:To investigate the antitumor effect of HuaZheng Koufuye on mice bearing H_ 22 and its possible mechanism.Methods: Seventy-five healthy mice were divided into 5 groups randomly:the normal group, the cancerbearing model group, the high dose group, the middle dose group and the positive group. Except the nomal group, the other 4 group were hypodermic injectel with oncocyte suspension 0.2ml per mouse.From the second day, the mice of the control group and the model group were given sodium chloride by gastrolavage. The mice of the high dose and low dose group were given by gastrolavage with 100% and 50% Huazheng Koufuye 10ml/kg,twice daily for ten day, respectively. The mice of the postive group were intraperitoneal injected with 10ml/kg CTX once in two days' time.The immunohistochemistry assay was used to detect the expression of p53 in tumor tissue, the radioimmunoassay was used to detect the level of ET in plasma of each group. Results: The level of p53 protein expression in the model group was much higher than the other 3 groups (P0.05),but there were no significant differences among the 3 groups. The ET content in the model group was the highest, while in the normal group was the lowerest, though the ET level reduced in the other 3 groups, the high dose group decrease obviousely and near to the normal group, there was great difference between high dose group and the other 2 groups still. (P0.05). Conclusion: Huazheng Koufuye can inhibit the tumor growth and reduce its vascular service perhapse by down-regulating the expression of mutant p53 and plasma ET content. This may be one its antitumor mechanisms.