The Impacts of Shizao Decoction to p53 Mutation and Expression of Endothelin in Tumor-bearing Mice

Objective Investigate the anti-tumor effect and the possible treatment mechanism of shizao decoction to mice bearing H22 with ascites tumor.Methods 75 healthy mice were randomly divided into 5 groups,which were the blank control group,tumor model group,positive control group and high and middle dose of Shizao Decoction group.Except the blank control group,the other mice of four groups were given subcutaneous injection of tumor cell suspension,0.2 mL/only to establish model.The next day after modeling,the blank control group and tumor model group were fed with normal saline(NS).The mice of high and middle dose group were given 100% and 50% concentration of Shizao Decoction to feed for 10 days,two times a day.The mice of positive control group were given CTX 10 mL/kg intraperitoneal injection for 5 times,Once every other day.Used immunohistochemical method to detect mutant p53 protein in vivo tumor and measured the levels of mouse plasma endothelin(ET)by radioimmunoassay.Results The expressing mutant p53 levels of tumor model group were significantly higher than the other three groups(P0.01),and three treatment groups have different degree of decline,but the difference among these three groups was not significant(P0.05).The plasma level of ET in tumor model group was significantly higher than that in blank control group(P0.01).All the dose groups,ET levels were decreased in different degrees,especially the high dose group that was close to the blank control group.Conclution Shizao Decoction can reduce the expression of mutant p53 protein,plasma ET levels and the tumor's blood supply.These may be one of the anti-tumor effect mechanisms of shizao decoction.