2013•Guizhou nongye kexueRequires access

Chromosome Karyotype Analysis of Crucian carp in Caohai

Zhou Qichun

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Abstract

In order to investigate the composition characteristics of the karyotype of C.carp in Caohai,chromosomes and karyotype of 35 C.carp in Caohai were analyzed by PHA and the body injection method of colchicine directly making the specimen of the chromosomes of the kidney cell.The results show that chromosomes of the kidney cell is composed of 150 elementary chromosomes and several small supernumerary chromosomes.The 150 chromosomes can be divided into four sections according to the position of the centromere,which are metacentric chromosome,submertace-ntric chromosme,subtelocentric chromosome and telocentric chromosome.There are 11 metacentric chromosomes,8 submetacen-tric chromosomes,11 subteocentric chromosomes and 20 telocentric chromosomes.All the chromosome groups are composed of three complete matched homologous chromosomes.These results indicated that C.carp in Caohai is a triploid,whose chromosome number is 3n=150+.The karyotype formula is 3n=33m+24sm+33st+60t,NF=207.

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In order to investigate the composition characteristics of the karyotype of C.carp in Caohai,chromosomes and karyotype of 35 C.carp in Caohai were analyzed by PHA and the body injection method of colchicine directly making the specimen of the chromosomes of the kidney cell.The results show that chromosomes of the kidney cell is composed of 150 elementary chromosomes and several small supernumerary chromosomes.The 150 chromosomes can be divided into four sections according to the position of the centromere,which are metacentric chromosome,submertace-ntric chromosme,subtelocentric chromosome and telocentric chromosome.There are 11 metacentric chromosomes,8 submetacen-tric chromosomes,11 subteocentric chromosomes and 20 telocentric chromosomes.All the chromosome groups are composed of three complete matched homologous chromosomes.These results indicated that C.carp in Caohai is a triploid,whose chromosome number is 3n=150+.The karyotype formula is 3n=33m+24sm+33st+60t,NF=207.

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Available abstract

In order to investigate the composition characteristics of the karyotype of C.carp in Caohai,chromosomes and karyotype of 35 C.carp in Caohai were analyzed by PHA and the body injection method of colchicine directly making the specimen of the chromosomes of the kidney cell.The results show that chromosomes of the kidney cell is composed of 150 elementary chromosomes and several small supernumerary chromosomes.The 150 chromosomes can be divided into four sections according to the position of the centromere,which are metacentric chromosome,submertace-ntric chromosme,subtelocentric chromosome and telocentric chromosome.There are 11 metacentric chromosomes,8 submetacen-tric chromosomes,11 subteocentric chromosomes and 20 telocentric chromosomes.All the chromosome groups are composed of three complete matched homologous chromosomes.These results indicated that C.carp in Caohai is a triploid,whose chromosome number is 3n=150+.The karyotype formula is 3n=33m+24sm+33st+60t,NF=207.

Key concepts: Karyotype, Biology, Chromosome, Microchromosome, Genetics, Small supernumerary marker chromosome, Centromere, Crucian carp

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