Resources evaluation and content of total flavonoids of leaves from Ilex kuding and relatives species

The content of total flavonoids from 5 species of Ilex L. used as kudingcha tea were determined using ultrasonic extraction, complexation reaction and UV spectrophotometry. On the average of value of total flavonoids from 5 batches of planting materials, Ilex kudingcha(3.96%)I.pentagona(3.67%)I. huoshanesis(3.63%)I.cornuta(3.04%)I. latifolia(2.64%); and total flavonoids in the leaves of placed after two months at low temperature dried has decreased significantly; On the average, the content of total flavone content of cultivation of mature leaves of I. kudingcha was significantly higher than that of wild mature leaves. The content of total flavonoids of I.huoshanesis was highest(7.39%) in all individuals from five species. Content of total flavonoids in young leaves maintained a higher level. The results showed that I.huoshanesis was a better germplasm resources, and it had a scientific significance that people used the tender leave as materials of Kudingcha tea.