Study on Preparation of Pea Peptide from Enzymatic Hydrolysis of Papain

Pea protein was hydrolyzed with papain. The effects of enzyme concentrate, substrate concentration, temperature,pH,time on enzymatic hydrolysis were discussed. The optimum condition for producing soluble peptides from pea protein by papain were found out through perpendicular experiment: concentrate is 8 % , substrate concentration is 7 %, 65 ℃, 3.5 h, pH 6.5. The molecular weight of the soluble peptide was determined by using High Performance Liquid Chromatography. The result showed that the degree of hgdrolysis reached 30 %,the molecular weight of 80 % soluble peptides was below 500 u.