Study on preparation of walnut peptides from enzymatic hydrolysis of pepsin and papain

Walnut peptides were obtained through pepsin or papain enzymolysis with walnut residue as raw materials and degree of hydrolysis as index.The results showed that papain was the optimum proteinase.The optimum hydrolytic conditions were as followed:substrate concentration was 4%,enzyme concentration was 5%,pH was 7,temperature was 50℃and hydrolysis time was 4h.Under these optimum conditions,the degree of hydrolysis can be higher than 25.76%.