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2007•Unpublished venueRequires access

Determination of paeoniflorin in Xianglian Huazhi Wan by HPLC

Yufang Li

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Abstract

OBJECTIVE To establish a HPLC method for the determination of Paeoniflorin in Xianglian Huazhi Wan.METHODS Kromasil C18 column(250 mm×4.6 mm,5 μm) was used with methanol-water(25∶75) as the mobile phase;The detection wavelength was 230 nm and the flow rate was 1.0 ml/min with the column temperature of 30 ℃.RESULTS The linear range was 14.24~85.44 μg/ml(r=0.999 7);The average recovery rate was 99.19%,RSD=1.28%.CONCLUSION The method was sensitive,accurate and reproducible.It is suitable for determining the content of Paeoniflorin in Xianglian Huazhi Wan.

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What this paper is about

OBJECTIVE To establish a HPLC method for the determination of Paeoniflorin in Xianglian Huazhi Wan.METHODS Kromasil C18 column(250 mm×4.6 mm,5 μm) was used with methanol-water(25∶75) as the mobile phase;The detection wavelength was 230 nm and the flow rate was 1.0 ml/min with the column temperature of 30 ℃.RESULTS The linear range was 14.24~85.44 μg/ml(r=0.999 7);The average recovery rate was 99.19%,RSD=1.28%.CONCLUSION The method was sensitive,accurate and reproducible.It is suitable for determining the content of Paeoniflorin in Xianglian Huazhi Wan.

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Available abstract

OBJECTIVE To establish a HPLC method for the determination of Paeoniflorin in Xianglian Huazhi Wan.METHODS Kromasil C18 column(250 mm×4.6 mm,5 μm) was used with methanol-water(25∶75) as the mobile phase;The detection wavelength was 230 nm and the flow rate was 1.0 ml/min with the column temperature of 30 ℃.RESULTS The linear range was 14.24~85.44 μg/ml(r=0.999 7);The average recovery rate was 99.19%,RSD=1.28%.CONCLUSION The method was sensitive,accurate and reproducible.It is suitable for determining the content of Paeoniflorin in Xianglian Huazhi Wan.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Linear range, Chemistry, Analytical Chemistry (journal), Detection limit

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