Comparison and evaluation of the self-established assay system for adenosine deaminase

Objective To evaluate the comparability and clinical acceptability of the results of serum adenosine deaminase(ADA) measured by self-established assay system with Beijing Leadman ADA kits in Roche Modular DPP clinical chemistry analyzers compared with traceable Roche Modular P reference assay system.Methods The concentrations of ADA in 40 fresh outpatient serum samples were analyzed in duplicate with two self-established systems and traceable Roche Modular reference system.The linear regression analysis was calculated according to National Committee for Clinical Laboratory Standards CLSI document EP9-A files.Results The comparability of serum ADA measurement results by self-established systems compared with Roche Modular reference system was acceptable.The regression equation was Y=0.998 7X+0.090 2,and the correlation coefficient(r2) was 0.998 4.Conclusion The results of serum ADA measured by our self-established systems have good comparability compared with Roche Modular P reference system,and it can replace the reference system to detect clinical specimens,which could be acceptable by clinical application.