Study of protection mechanism of aralosides on myocardial ischemia/reperfusion injury in rats

Objective: To investigate the effects of aralosides on expression of inflammatory molecule ICAM-1 and the activity of superoxide dismutase(SOD) in rats after myocardial ischemia/reperfusion(MI/R) injury.Methods: A model of myocardial ischemia 45min followed by 6h reperfusion was made by reversibly ligating coronary left descending artery.30 Wistar rats were divided randomly into 3 groups: ischemia reperfusion group,aralosides group,Plavix group.HE staining was used to observe the pathological changes of myocardic tissues.The activity of SOD was measured too.ELISA was used to determine sICAM-1 expression in myocardium.Results: In rats treated by aralosides or Plavix,the lactate dehydrogenase(LDH) activity was significantly decreased.Meamwhile,Aralosides and Plavix raised the activity of superoxide dismutase(SOD).ICAM-1 expression was detected in myocardium after ischemia 45min followed by 6h reperfusion.In rats treated by aralosides or Plavix,ICAM-1 expression remained unchanged.Conclusion: Aralosides could protect myocardium from inflammatory injury during MI/R,which may be achieved by increasing the activity of SOD,confronting the toxic action of oxygen radical to cardiocytes and decreasing the injury of cardiocytes.