Function of Exogenous Hormones on Maize Tissue Culture in Vitro
Gao Wujun
Abstract
Gao Wujun
Abstract
The young embryos of maize inbred lines Huangzao4,Zong31 were cultured in vitro and the important functions of plant hormones were studied.The results indicated that medium for high frequency embryogenic callus was MS+2.0 mg/L 2,4-D;MS+6-BA 0.5 mg/L+KT(1.0)mg/L+NAA 0.2 mg/L was better for the callus differentiation;MS+1.0mg/L NAA was good for root development and got almost the same survival rate as MS+NAA1.0 mg/L+MET(2.0)mg/L did.
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The young embryos of maize inbred lines Huangzao4,Zong31 were cultured in vitro and the important functions of plant hormones were studied.The results indicated that medium for high frequency embryogenic callus was MS+2.0 mg/L 2,4-D;MS+6-BA 0.5 mg/L+KT(1.0)mg/L+NAA 0.2 mg/L was better for the callus differentiation;MS+1.0mg/L NAA was good for root development and got almost the same survival rate as MS+NAA1.0 mg/L+MET(2.0)mg/L did.
Key concepts: Callus, In vitro, Hormone, Tissue culture, Embryo, Biology, Inbred strain, Botany