Regeneration System and Influencing Factors of Maize in Ningxia

Using immature embryo of four maize varieties in Ningxia as explants and utilizing the plant tissue culture method,the effect of medium type,genotype,exogenous hormone and cephamycin(Cef) on in vitro culture and regeneration was studied.The results showed that different medium type and genotype showed different ability of embryogenic callus inducing.Ⅰand Ⅱ had more high rate of induction,Ningyu 12 and Ning 0816 had high rate as 85.3% and 82.1% respectively;6-BA were the most important factor for the induction of differentiation of embryogenic callus,6-BA could promote the differentiation of embryogenic callus.Respectively,when 500 mg/L of Cef was added,the differentiation of callus was significant inhibited.The best inducing medium was N6 +Casein hydrolysate 200 mg/L+Pro 690 mg/L+2,4-D 2.0 mg/L + Sugar 30 g/L+Inositol 100 mg/L+AgNO310 mg/L,The best differentiation medium was: N6+6-BA1.0 mg/L+Sugar 50 g/L.