Production of Difloxacin Antibody

[Objective] The research aimed to difloxacin(DIF) antibody.[Method] Using N-hydroxysuccinimide active ester(NHS) method,difloxacin was coupled with bovine serum albumin(BSA) and ovalbumin(OVA) to prepare the immunizing antigen DIF-BSA and detecting antigen DIF-OVA.Mice were immunized by using DIF-BSA to obtain the antibody with high titer.[Result] The ultraviolet spectrum of immunizing antigen DIF-BSA had the stacking characteristics of medicament and protein and its maximum absorption peak appeared at the wavelength of 277 nm,which indicated that vector protein BSA was successfully coupled with DIF and it could be used in animal immunization.The maximum absorption peak of detecting antigen DIF-OVA appeared at the wavelength of 276 nm,indicating vector protein OVA was successfully coupled with DIF and it could be taken as the detecting antigen for coating.The results of indirect ELISA detection showed that the antiserum titers of mice were higher and over 1:32 000,which indicated that the specific antibody was produced.[Conclusion] The polyclonal antibody against DIF was successfully prepared,which further proved that DIF were successfully coupled.