Experimental Study on the Relations between the TGF-β_1/Smad and Different TCM Syndromes of Liver Fibrosis

Objective:To observe the relations between the TGF-β1 /Smad and different TCM syndromes of liver fibrosis.Methods:All rats of model groups were treated by CCl4to establish the liver fibrosis models.Besides CCl4,the rats of liver-stagnation and spleen-deficiency group were treated by stimulating tails with forceps clip and gastric infusion of rhubarb decoction,and the rats of qi-deficiency and blood-stasis group were treated by the method of swimming-fatigue.Then every group was intervened by the relevant medicine.After all treatments,the TGF-β1,TβR-Ⅰ,Smad2/3,Smad7 and Smad3mRNA of liver were examined.Results:The TGF-β1,TβR-Ⅰ,Smad2/3,Smad7 and Smad3mRNAof model groups were expressed significantly(P 0.01) .The expression of qi-deficiency and blood-stasis group is the most significant.The expressions of liver-stagnation and spleen-deficiency group and pure liver fibrosis group are similar.Conclusions:The signal transduction pathway of TGF-β1 /Smad participate in the occurrence and development of liver fibrosis.There are differences of TGF-β1 /Smad expression among different TCM syndrome groups.