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2003•Journal of Oceanography in Taiwan StraitRequires access

Impacts of Vibrio alginolyticus lipopolysaccharide(LPS)on toxicity and immune protection of Pseudosciaena crocea

Yan Qing

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Abstract

97.6 mg LPS were obtained by phenol water method from 20dm 3 suspension of Vibrio alginolyticus ,the pathogenic bacteria of Pseudosciaena crocea. Toxicity of LPS from V.alginolyticus on P.crocea was examined by intramuscular injection.The results showed that injecting 0.5mg/cm 3 LPS would lead to the death of P.croceain 5d, and the mortality rate of P.crocea would reach 100% when the concentration of LPS raised to 2.0mg/cm 3. 15d after injection of LPS (0.2mg/cm 3) 0.2cm 3 to P.crocea, 40% of P.crocea were immunely protected when attacked by 0.2cm 3 V.alginolyticus suspension cells (9×10 8ind./cm 3).

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What this paper is about

97.6 mg LPS were obtained by phenol water method from 20dm 3 suspension of Vibrio alginolyticus ,the pathogenic bacteria of Pseudosciaena crocea. Toxicity of LPS from V.alginolyticus on P.crocea was examined by intramuscular injection.The results showed that injecting 0.5mg/cm 3 LPS would lead to the death of P.croceain 5d, and the mortality rate of P.crocea would reach 100% when the concentration of LPS raised to 2.0mg/cm 3. 15d after injection of LPS (0.2mg/cm 3) 0.2cm 3 to P.crocea, 40% of P.crocea were immunely protected when attacked by 0.2cm 3 V.alginolyticus suspension cells (9×10 8ind./cm 3).

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Available abstract

97.6 mg LPS were obtained by phenol water method from 20dm 3 suspension of Vibrio alginolyticus ,the pathogenic bacteria of Pseudosciaena crocea. Toxicity of LPS from V.alginolyticus on P.crocea was examined by intramuscular injection.The results showed that injecting 0.5mg/cm 3 LPS would lead to the death of P.croceain 5d, and the mortality rate of P.crocea would reach 100% when the concentration of LPS raised to 2.0mg/cm 3. 15d after injection of LPS (0.2mg/cm 3) 0.2cm 3 to P.crocea, 40% of P.crocea were immunely protected when attacked by 0.2cm 3 V.alginolyticus suspension cells (9×10 8ind./cm 3).

Key concepts: Vibrio alginolyticus, Lipopolysaccharide, Microbiology, Toxicity, Chemistry, Biology, Bacteria, Vibrio

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Impacts of Vibrio alginolyticus lipopolysaccharide(LPS)on toxicity and immune protection of Pseudosciaena crocea — Research Paper | ScholarLens