“Huoxue Qiangyang Formula”on Insulin Resistance and Insulin-signaling Pathway in Spontaneous Hypertensive Rats of Blood Stasis,Yang Hyperactivity,Turbid Phlegm Pattern

Objective: To explore the effect of Huoxue Qiangyang Formulain treating hypertension and improving insulin resistance and its possible mechanism. Methods: Rats were randomly divided into three groups: SHR control group, model group and treatment group. Another WKY rats were taken as normal control group. The rats in treatment group were treated withHuoxue Qiangyang Formula,and rats in the other groups were treated with normal saline,with the course of 8 weeks. We detected the blood pressure,blood fat,blood glucose,insulin metabolism in each group. The mRNA expression of insulin signal pathway was detected by RT-PCR and related protein was verified by Western Blotting. Results: The levels of BP,TG,TC,FPG,FIN and IRI of treatment group were significantly lower than that of the model group( P 0. 01); the expression of GCK mRNA and G6PC mRNA in treatment group were significantly up-regulated( P 0. 01),and the Pdk4 expression was significantly down-regulated( P 0. 05). Conclusion: Huoxue Qiangyang Formulacan improve the BP, lipid levels and insulin resistance in rats with blood stasis-yang hyperactivity-turbid phlegm pattern. Its mechanism may be association with up-regulation of GCK and G6PC,and down-regulation of Pdk4 of insulin signal pathway.