Effect of Lycopene on Mitochondrial Damage of PC12 Cells Induced by Rotenone

Objective: To investigate the protective effects of lycopene against rotenone-induced mitochondrial damage in PC12 cells. Method: All the experiments were carried out with PC12 in vitro,which were divided into 5 group,control,model( 0. 5 μmol·L- 1),lycopene( 3,10,30 μmol·L- 1) preincubation.Cell viability was determined by CCK-8 assay; inverted phase contrast microscope was used to observe the cell morphological was; the ultrastructural changes of neuronal mitochondria were viewed under transmission electron microscope. The flow cytometry was used to detect the mitochondrial membrane potential( MMP). Result: CCK-8assay showed that rotenone had cytotoxicity in PC12 cells,compared with the cells in the control group,cell viability was declined to 70. 34% ± 2. 81%( P 0. 05),the pre-treatment of lycopene( 3,10,30 μmol·L- 1)could significantly increase the PCI2 cell viability,cell viability was increased to 83. 09% ± 3. 15%,87. 24% ±2. 15%,89. 17% ± 2. 26%( P 0. 05). Compared with the cells in the model group, the number and morphology of neuronal mitochondria changed distinctly in the lycopene pretreated cells. Compared with the cells in the model group,the pre-treatment of lycopene( 3,10,30 μmol·L- 1) increased mean fluorescence intensity of mitochondrial membrane potential to 202. 24 ± 26. 28, 226. 21 ± 9. 71, 238. 83 ± 10. 29( P 0. 05).Conclusion: Lycopene can exert protective effects against rotenone-induced mitochondrial damage in PC12 cells.