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2010•Guangdong nongye kexueOpen access

Determination of trans fatty acids in edible oil by gas hromatography

Lan Guo-dong

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Abstract

After converted to methyl esters with KOH-CH3OH, trans fatty acids in edible oil were separated and analyzed by capillary gas (DB-23) chromatography with high polarity. The results showed that a good linear relationship (r=0.999)existed in the range of 0～2.0 mg/mL under optimal conditions and the detection limit were10 μg/mL (S/N=3). The recoveries ranged from 90.2% to 98.0%, with the relative standard deviations of 1.5%～4.1%. The method was shown to be simple, convenient and accurate and was suitable for the determination of trans fatty acids in edible oil.

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What this paper is about

After converted to methyl esters with KOH-CH3OH, trans fatty acids in edible oil were separated and analyzed by capillary gas (DB-23) chromatography with high polarity. The results showed that a good linear relationship (r=0.999)existed in the range of 0～2.0 mg/mL under optimal conditions and the detection limit were10 μg/mL (S/N=3). The recoveries ranged from 90.2% to 98.0%, with the relative standard deviations of 1.5%～4.1%. The method was shown to be simple, convenient and accurate and was suitable for the determination of trans fatty acids in edible oil.

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Available abstract

After converted to methyl esters with KOH-CH3OH, trans fatty acids in edible oil were separated and analyzed by capillary gas (DB-23) chromatography with high polarity. The results showed that a good linear relationship (r=0.999)existed in the range of 0～2.0 mg/mL under optimal conditions and the detection limit were10 μg/mL (S/N=3). The recoveries ranged from 90.2% to 98.0%, with the relative standard deviations of 1.5%～4.1%. The method was shown to be simple, convenient and accurate and was suitable for the determination of trans fatty acids in edible oil.

Key concepts: Chemistry, Chromatography, Detection limit, Gas chromatography, Edible oil, Capillary gas chromatography, Linear relationship, Relative standard deviation

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