Inhibitory effect of grape procyanidin on abnormal expression of parp mRNA in rat's hepatic cells

ObjectiveTo study the effect of grape procyanidin (GPC) on N-nitroso compounds-induced expression of poly (ADP-ribose) polymerase (PARP) mRNA in rat's hepatic cells. Methods0.5% NaNO\-2 was infused into Wistar rats to indu ce hepatocytes' mutation,and GPC was infused by 100 and 10?mg/kg respectively t hrough mouth of the two experimental groups at the same time.After eight weeks,h epatic tissue was fixed in vivo and situ hybridization was applied to measur e the expression level of PARP mRNA in rats' hepatic cells,and its expression le vel was quantitatively analyzed by an image analysis instrument. ResultsThe positive cells rate of PARP mRNA expressed in the induced-injury group was 30 12% and optical density was 0.354?3±0.041?9 ,and those of the high-dose GPC group were 11.48% and 0.268?7± 0.017?2 ,and the difference between the two groups had statistical significance(P0.01). ConclusionGPC had inhibitory effect on N-nitroso compoun ds-induced abnormal expression of PARP mRNA in hepatic cells of rats.