A Primary Analysis on Differential Expression of Proteins in Diapause and Non-diapause Pupae of Antheraea pernyi

Chinese oak silkworm(Antheraea pernyi) is one of the insect species that hibernate in form of diapause pupa.Understanding the variation regularity of protein varieties and contents in diapause and non-diapause pupae would facilitate clarification of diapause mechanism.SDS-PAGE results showed that there were 25 clear protein bands isolated from both diapause and non-diapause male pupae of A.pernyi,among which proteins of 79,54,48,and 29 kD had high expression levels and the 79 kD protein was a high molecular weight protein abundantly expressed.Two dimension electrophoresis(2D-PAGE) results showed that the detected number of total protein spots from protein sample of diapauses pupae was 400,and that of non-diapause pupae was 619.There were obviously more protein spots from non-diapause pupae.The matching rate of protein spots between them was only 37%.Isoelectric points of these proteins were greatly varied,with big difference and low matching rate.These experimental results suggest that protein varieties and contents in A.pernyi pupae have remarkable changes in the process of diapause.