A Preliminary Study on the Content and Electrophoresis Patterns of Soluble Protein in Hemolymph from Diapause and Non-diapause Pupae of Helicoverpa Assulta

The content and composition of soluble protein in the hemolymph from diapause and non-diapause pupae of Helicoverpa assulta were measured and analyzed by using Bradford and SDS-PAGE.The results showed that,the change tendency of soluble protein content in the non-diapause was down-up-domn from pupation to eclosion,and the largest content appeared on the fifth day after pupation,but the content in the diapause pupae decreased continuously.Except for 2-day female pupae,there were significant differences in the soluble protein content between the female and male pupae.The electrophoretograms of SDS-PAGE indicated that there were signficant differences in the composition and content of soluble protein between diapause and non-diapause pupae.So it could be deduced that these changes of soluble protein of hemolymph in the diapause pupae were related to the cold-resistance of H.assulta.