Experimental study of the TNF-α of Rats in severe acute pancreatitis with acute Renal Injury

Objective To explore the expressive changes of TNF-amRNA in severe acute pancreatitis complicated with acute Renal Injury (ARI) and its mechanisms. Methods 32 healthy SD rats were randomly divided into two groups:sham operation group (n=16),pancreatitis model group (n=16). sham operation group: open rats abdomen and only tipping intestinal canal;the model of severe acute pancreatitis was induced by retrograde injection of 3% Sodium Taurocholate into bilio-pancreatic duct。 All rats were sacrificed after the SAP model had been produced at 12 hours and 24 hours .The rats blood was drawn from portal vein and detected the Concentrations of blood serum –amylase,blood serum-urea nitrogen、creatinine and the expression of renal tissue TNF-a mRNA. Pathologic change of kidney and pancreas were observed under light microscope. Results The concentrations of blood serum amylase,blood serum -urea nitrogen、creatinine and the expression of renal tissue TNF-a mRNA were higher in pancreatitis Group than that in sham operation Group. pathological lesion were server in pancreatitis Group, ascetic and specifications were emerged in pancreatitis Group. sham operation group was normal. Conclusions The expression of renal tissue TNF-αmRNA and alleviate the pathologic damage of the pancreas and kidney in rats SAP. it is a cute that the TNF-αmRNA of renal tissue play a very important etiopathogenisis role in rats SAP complicated with ARI。