The protective effect of dioscin-containing serum on hydrogen peroxide injured cardiomyocytes of neonate rats

OBJECTIVE To study the effects and mechanism of dioscin on hydrogen peroxide(H2O2) injured cardiomyocytes with a serum pharmacological method.METHODS Prepared the serum containing dioscin(600 mg·kg-1) with serum pharmacological method.Cardiomyocytes derived from neonatal rats were cultured in Dulbecco Modified Eagle Medium(DMEM).The cardiomyocytes were injured with hydrogen peroxide(H2O2),and treated with the serum containing different concerntrations of dioscin.The survival rates of cardiomyocytes were measured with MTT method.The contents of malondialdehyde(MDA) and lactate dehydrogenase(LDH),and the activities of total superoxide dismutase(T-SOD) and catalase(CAT) were measured.RESULTS The survival rates of cardiomyocytes injured with H2O2 for 2 h were decreased significantly compared with normal cells(P0.01).The content of MDA and LDH were increased significantly,but the activity of T-SOD and CAT were decreased.After treated with the different concentrations of drug-containing serum,the survival rates were increased with a dose-dependant manner(P0.01).The contents of MDA and LDH were decreased and the activities of SOD and CAT were significantly increased(P0.01).CONCLUSION Dioscin can protect the H2O2 injured cardiomyocytes by increasing antioxidation capability.