Effect of Tremella Polysaccharides on cardiomyocytes in vitro and in vivo

Objective To observe the anti-apoptosis effects of Tremella Polysaccharides(TP) on cardiomyocytes in vitro and in vivo.Methods Cardiomyocytes were separated from neonate rats and were cultured in vitro.The cells were assigned to normal control group,apoptosis-induced group,and TP-pretreated group randomly. The morphology,Na~+-K~+-ATPase activity,and apoptosis index of cardiomyocytes were studied after 72 hours. In the intact animal study,100 ICR mice were included and assigned randomly into 5 grouPs after paired by body weight:negative control group(peritoneal injected by saline daily);positive control group(peritoneal injected by D-galactose and intragastric administrated with saline daily),and three TP-pretreated group groups(peritoneal injected by D-galactose and intragastric administered with 100,200,400 mg/kg TP respectively).Mice were sacrificed for histology,apoptosis index,and antioxidation studies after 8 weeks.Results Na~+-K~+-ATPase activity of cardiomyocytes were as follows:normal control groupTP-pretreated groupapoptosis-induced group(P0.05);apoptosis index:apoptosis-induced groupTP-pretreated groupnormal control group(P0.01).In the animal studies,there was no significant difference in myocardium pathology among groups.The apoptosis index detected by TUNEL was 400 mg/kg TP group200 mg/kg TP grouppositive control group(P0.05).Myocardium homogenate detection showed that malondialdehyde(MDA) and lipofuscin(LP) in 200 and 400 mg/kg TP groups were significantly lower than in positive control group(P0.05),MDA and LP in 400 mg/kg TP group was significantly lower than in 200 mg/kg TP group(P0.05);and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase(SOD) in all TP-pretreating groups were significantly higher than in positive control group(P0.05);among which the 400 mg/kg TP group had the highest activities of GSH-Px and SOD (P0.05).Conclusion TP can suppress the apoptosis of cardiomyocytes induced by oxidative damage in vitro, indicating that TP has protective effect on cardiomyocytes.In aging mice induced by D-galactose experiment,TP has anti-apoptosis and anti-oxidation effect in a dose-dependant manner.