Effects of fenofibrate on proliferation and apoptosis induced by lysophosphatidylcholine in cultured human umbilical vein endothelial cells

AIM: To investigate the effect of fenofibrate on proliferation and apoptosis induced by lysophosphatidylcholine(LPC) in cultured human umbilical vein endothelial cells (HUVECs) and its mechanism. METHODS: HUVECs were cultured in vitro. The study was designated to 5 groups:normal control;group LPC;low-concentration fenofibrate(10 μmol·L~ -1 )group; middle- concentration fenofibrate (50 μmol·L~ -1 )group;high- concentration fenofibrate(100 μmol·L~ -1 )group. Proliferation and apoptosis of HUVECs were evaluated by MTT assay, flow cytometry (FCM) and fluorescence microscopy. The expression of XIAP protein was examined by immunohistochemistry method. RESULTS: Compared with control group, LPC could inhibit the growth and induce apoptosis of HUVECs, and decrease the expression of XIAP protein in HUVECs. Fenofibrate could increase the growth and decrease the apoptosis of HUVECs, and enhance the expression of XIAP protein in HUVECs. CONCLUSION: Fenofibrate can improve proliferation and apoptosis induced by lysophosphatidylcholine in HUVECs by enhancing expression of XIAP protein.